Optimized Sample Preparation and Microscale Separation Methods for High-Sensitivity Analysis of Hydrophilic Peptides
The optimization of solid-phase extraction (SPE) purification Universal Drain Hose and chromatographic separation is usually neglected during proteomics studies.However, the effects on detection performance are not negligible, especially when working with highly glycosylated samples.We performed a comparative study of different SPE setups, includin